Package: ribiosNGS Type: Package Title: Differential gene expression analysis for next-generation sequencing data in the 'ribios' suite Version: 1.0.0 Authors@R: c(person(given = "Jitao David", family = "Zhang", role = c("aut", "cre", "ctb"), email = "jitao_david.zhang@roche.com", comment = c(ORCID="0000-0002-3085-0909")), person(given="Ailu", family="Mading", role="ctb")) Description: Provides a comprehensive workflow for differential gene expression analysis of RNA-seq data using 'edgeR' and 'limma'-voom pipelines. The package supports count filtering, normalization, surrogate variable analysis, batch correction, and visualization including volcano plots, smear plots, and PCA. It integrates with the 'ribios' suite of packages and is designed for reproducible bioinformatics analyses. Depends: R (>= 4.4.0), Imports: BiocGenerics, ribiosExpression (>= 1.2.0), edgeR, ribiosAnnotation (>= 3.6.0), ribiosUtils, methods, Biobase, lattice, ribiosIO (>= 1.0-60), ribiosPlot (>= 1.2-9), graphics, limma, readr, readxl, magrittr, sva, dplyr, vsn, made4, ggplot2, rlang, gt, scales, UpSetR, ggrepel, reshape2 Suggests: testthat, ggpubr, tibble, knitr, rmarkdown, BiocStyle biocViews: DifferentialExpression, GeneExpression, RNASeq, Transcriptomics License: GPL-3 VignetteBuilder: knitr Encoding: UTF-8 RoxygenNote: 7.3.3 Collate: 'utils.R' 'ribiosNGS-package.R' 'AllClasses.R' 'AllGenerics.R' 'AllMethods.R' '20210217-toribios.R' 'DGEListToLongTable.R' 'EdgeObject.R' 'annotateMethods.R' 'pseudoT.R' 'appendZScore.R' 'edgeR-funcs.R' 'dge.R' 'dgeTable.R' 'edgeR-slurm-lsf.R' 'eset2DGEList.R' 'exportEdgeResult.R' 'filterByCPM.R' 'knockdown.R' 'logFCmatrix.R' 'logFCpca.R' 'mergeDGEList.R' 'minGroupCount.R' 'mps-io.R' 'normalize.R' 'pca.R' 'plotMethods.R' 'plotTopSigGenes.R' 'readBiokitAsDGEList.R' 'readFeatureAnnotationForEdgeR.R' 'readSampleAnnotationForEdgeR.R' 'renameContrast.R' 'split.DGEList.R' 'staticGeneLevelPlots.R' 'sva.R' 'svaseqRemove.R' 'topDgeExpression.R' 'tpm.R' 'voomLimma.R' 'writeBiokitSampleAnnotation.R' 'writeDGEList.R' Remotes: github::bedapub/ribiosExpression, github::bedapub/ribiosAnnotation, github::bedapub/ribiosUtils, github::bedapub/ribiosIO, github::bedapub/ribiosPlot Additional_repositories: https://bedapub.r-universe.dev URL: https://github.com/bedapub/ribiosNGS BugReports: https://github.com/bedapub/ribiosNGS/issues Config/pak/sysreqs: cmake make libicu-dev libpng-dev libuv1-dev libxml2-dev libssl-dev perl libsasl2-dev libnode-dev libx11-dev zlib1g-dev Repository: https://bedapub.r-universe.dev Date/Publication: 2026-02-15 11:40:09 UTC RemoteUrl: https://github.com/bedapub/ribiosNGS RemoteRef: HEAD RemoteSha: 90513e37c7ab00a23dff38258a29b2e7e4589443 NeedsCompilation: no Packaged: 2026-06-15 16:05:26 UTC; root Author: Jitao David Zhang [aut, cre, ctb] (ORCID: ), Ailu Mading [ctb] Maintainer: Jitao David Zhang